Análisis molecular y genético de mecanismos implicados en el aclaramiento de β-amiloide a través de los plexos coroideos en la enfermedad de Alzheimer

Tesis inédita de la Universidad Complutense de Madrid, Facultad de Ciencias Biológicas, Departamento de Bioquímica y Biología Molecular, leída el 06/04/2010.Sección Deptal. de Bioquímica y Biología Molecular (Biológicas)Fac. de Ciencias BiológicasTRUEProQuestpu

Baixos níveis séricos de vitamina D aumentam significativamente o risco de morte em idosos com fraturas de quadril : coorte prospectiva

Objective: to evaluate the relationship between 25(OH)D3 levels and fatal outcome in patients over 60 years of age undergoing surgical repair of hip fractures. Methods: prospective cohort of patients undergoing surgical repair of hip fractures. At admission, 25(OH)D3 levels were measured, among other parameters. Patients were followed for at least 1 year, and incident mortality was recorded. Results: 209 patients were included in the study, with a mean age of 79.5 ± 7.6 years among survivors and 80.7 ± 8.2 years among those who died in the first postoperative year (p=0.346). The 25(OH)D3 levels of survivors were significantly higher than those of patients who died (p=0.003). After adjusting for confounding variables, 25(OH)D3 levels below 12.5ng/mL were significant risk factors regardless of mortality (adjusted OR: 7.6; 95% CI: 2.35 to 24.56). Conclusions: our data show that serum 25(OH)D3 levels below 12.5ng/mL significantly and independently increased the risk of mortality in the first year after surgical repair of low-energy hip fracture in patients over 60 years of age in the geographic region where this study was conducted. Low albumin also showed a significant association with mortality in these patients. All other factors had no significant associations.Métodos: coorte prospectiva de pacientes submetidos a correção cirúrgica de fraturas de quadril. À admissão, foram medidos os níveis de 25(OH)D3, entre outros parâmetros. Os pacientes foram acompanhados por, pelo menos, um ano e a mortalidade foi registrada. Resultados: foram incluídos 209 pacientes no estudo, com média de idade de 79,5 ± 7,6 anos entre os sobreviventes e 80,7 ± 8,2 anos entre os que morreram no primeiro ano de pós-operatório (p=0,346). Os níveis de 25(OH)D3 dos sobreviventes foram significativamente maiores do que os dos pacientes que morreram (p=0,003). Após o ajuste para variáveis de confusão, níveis de 25(OH)D3 abaixo de 12,5ng/mL foram fator de risco significativo, independentemente da mortalidade (OR ajustado 7,6; IC 95% 2,35 24,56). Conclusões: níveis séricos de 25(OH)D3 abaixo de 12,5ng/mL aumentaram significativa e independentemente o risco de morte no primeiro ano após o reparo cirúrgico de fratura de quadril de baixa energia em pacientes com mais de 60 anos de idade na região geográfica onde este estudo foi realizado. A albumina baixa também teve associação significativa com a mortalidade nesses pacientes. Todos os outros fatores não tiveram associações significativas

Plant species influence on soil C after afforestation of Mediterranean degraded soils

6 figuras.-- Poster presentado en Geophysical Research Abstracts Vol. 17, EGU2015-12121, 2015Increasing C sequestration in terrestrial ecosystems is one of the main current environmental challenges to mitigate climate change. Afforestation of degraded and contaminated lands is one of the key strategies to achieve an increase in C sequestration in ecosystems. Plant species differ in their mechanisms of C-fixation, C allocation into different plant organs, and interaction with soil microorganisms, all these factors influencing the dynamics of soil C following the afforestation of degraded soils. In this work we examine the influence of different woody plant species on soil C dynamics in degraded and afforested Mediterranean soils. The soils were former agricultural lands that were polluted by a mining accident and later afforested with different native plant species. We analysed the effect of four of these species (Olea europaea var. sylvestris Brot., Populus alba L., Pistacia lentiscus L. and Retama sphaerocarpa (L.) Boiss.) on different soil C fractions, soil nutrient availability, microbial activity (soil enzyme activities) and soil CO2 fluxes 15 years after the establishment of the plantations. Results suggest that the influence of the planted trees and shrubs is still limited, being more pronounced in the more acidic and nutrient-poor soils. Litter accumulation varied among species, with the highest C accumulated in the litter under the deciduous species (Populus alba L.). No differences were observed in the amount of total soil organic C among the studied species, or in the concentrations of phenols and sugars in the dissolved organic C (DOC), which might have indicated differences in the biodegradability of the DOC. Microbial biomass and activity was highly influenced by soil pH, and plant species had a significant influence on soil pH in the more acidic site. Soil CO2 fluxes were more influenced by the plant species than total soil C content. Our results suggest that changes in total soil C stocks after the afforestation of degraded Mediterranean soils are hardly detectable at decadal time-scales, and that more dynamic pools and fluxes must be monitored to determine which plant species should be promote to enhance C sequestration capacity.The RECARE project is funded by the European Commission FP7 Programme, ENV.2013.6.2-4 "Sustainable land care in Europe". EU grant agreement: 603498.Peer reviewe

Análise dos extremos termo-higrométricos em Campo Grande - MS entre 2002 e 2017

O conhecimento do clima local constitui um importante fator para o estudo do ambiente, bem como a busca de melhor qualidade de vida para a população. Neste sentido, o presente artigo tem o objetivo de elencar e compreender a ocorrência de extremos termo-higrométricos em Campo Grande - MS. Para atingir o objetivo proposto, foram solicitados os dados da Estação Meteorológica de Campo Grande ao Instituto Nacional de Meteorologia. De posse desses dados, eles foram tabulados, classificados e, a partir daí, foram elencados os extremos termo-higrométricos para análise e compreensão dos eventos em questão. Entre os resultados obtidos, vale destacar que, na classificação dos anos-padrão, o ano de 2002 pode ser considerado como quente/seco ou alta amplitude termo-higrométrica dentro da série histórica analisada; em contraponto, o ano de 2009 caracterizou-se por ser frio/úmido e com baixa amplitude térmica e o ano de 2010 foi classificado como habitual em seis das oito variáveis analisadas. A partir das análises dos extremos de temperatura e umidade relativa, torna-se possível traçar indicadores que demonstram a influência dos fatores climáticos estudados no cotidiano da população na área de estudo, podendo, assim, nortear as políticas públicas e as ações da sociedade no intuito de minimizar, adaptar e/ou mitigar os impactos gerados pelos extremos climáticos analisados

Aplicación móvil basada en realidad aumentada para mejorar el aprendizaje de Historia del Perú en estudiantes de secundaria, Trujillo 2019

El siguiente trabajo de investigación denominado “Aplicación móvil basada en Realidad Aumentada para mejorar el Aprendizaje de Historia del Perú en estudiantes de secundaria, Trujillo 2019”, tiene como finalidad mejorar el aprendizaje del curso de Historia del Perú en los alumnos de primer grado de secundaria de la Institución Educativa 80756 José María Arguedas del distrito de El Porvenir. Se trabajó con las secciones del primer grado A y B que cuentan con 25 estudiantes cada sección, se obtuvo información por medio de encuestas a los estudiantes y padres de familia, también se usó la observación del registro de calificaciones; puesto que la población fue menor a 30, para el análisis de datos se usó la prueba t-student; El tipo de investigación es aplicada, el diseño de esta investigación es cuasi experimental con post test únicamente ya que se trabajó con un grupo de control y grupo de estudio; para el desarrollo del producto se utilizó la metodología ágil Scrum. Al usar la aplicación en el desarrollo de las clases, se incrementó en un 21% en el grado de interés de los alumnos en temas de cultura e identidad nacional, así mismo se elevó en un 11% el promedio de las calificaciones de los estudiantes en el curso de Historia del Perú, también se logró aumentar en un 29.8% el grado de satisfacción de los padres en relación al aprendizaje de sus hijos

Calidad de vida en pacientes con cáncer avanzado según lugar de atención en un hospital de referencia.: Quality of life in patients with advanced cancer according to place of care in a referral hospital.

The quality of life perceived by patients with advanced cancer from a tertiary hospital is compared according to home care versus hospital care. Observational, cross-sectional study, included patients older than 18 years with cancer with multiple metastases. EORTC QLQ-C15-PAL quality of life survey was applied to patients with adequate cognitive level (Pfeiffer questionnaire). Chi square and Mann-Whitney, p <0.05, and SPSS 24.0 were used. 83 patients in the hospital group and 69 at home. Median age 66 and 76 years respectively (p <0.01); the female sex predominated (p = 0.60). They presented moderate or severe cognitive impairment, 25% hospital group and 58% home (p <0.01). The median quality of life score was 49.5 in the hospital group and 48 in the home group (p = 0.60), the physical functional aspect had a lower score in the hospital group (p <0.01). The perceived quality of life is acceptable, the home group is older and has cognitive impairment.Se compara la calidad de vida percibida por pacientes con cáncer avanzado de un hospital terciario según atención en domicilio versus hospitalaria. Estudio observacional, transversal, incluyó pacientes mayores de 18 años con cáncer con metástasis múltiple. Se aplicó encuesta de calidad de vida EORTC QLQ-C15-PAL a los pacientes con adecuado nivel cognitivo (cuestionario de Pfeiffer). Se utilizó Chi cuadrado y Mann-Whitney, p<0,05, y SPSS 24.0. 83 pacientes grupo hospitalario y 69 domiciliario. Mediana de edad 66 años y 76 respectivamente (p<0,01); predominó el sexo femenino (p=0,60). Presentó deterioro cognitivo moderado o severo, 25% grupo hospitalario y 58% domiciliario (p<0,01). La mediana del puntaje de calidad de vida fue 49,5 grupo hospitalario y 48 domiciliario (p=0,60), el aspecto funcional físico tuvo menor puntuación en grupo hospitalario (p<0,01).La calidad de vida percibida es aceptable, el grupo domiciliario tienen mayor edad y deterioro cognitivo

Trends and time variability in the northern continental shelf of the western Mediterranean

18 pages, 7 figures, 1 table, supporting information https://doi.org/10.1029/2004JC002799Different works appearing during the last decade show that the western Mediterranean has suffered a temperature and salinity increase during the 20th century. Most of these works analyze long-term trends in Levantine Intermediate Water (LIW) and Western Mediterranean Deep Water (WMDW); those dealing with changes in shallow and coastal waters are very scarce. It is still an open question whether these changes are due to meteorological factors occurring in the western Mediterranean or if they are caused by the salinity increase of the water masses contributing to deep water formation. In this work we analyze the data obtained in the last decade of the 20th century (and longer time series in some cases) within the frame of six projects aimed at the systematic collection of hydrographic data at fixed stations in the northern sector of the western Mediterranean (mainly in coastal areas). We detect strong warming trends in those stations located in the continental shelf (and probably with no influence of the LIW). This result could indicate that changes observed in the WMDW cannot be explained only on the basis of changes imported from the eastern basin. Another striking result is that these trends are an order of magnitude higher than those reported for the rest of the century, indicating that the 1990s have been an exceptionally warm decade. On the other hand, time series affected by the LIW show a salinity increase, and in some cases this is not accompanied by the corresponding temperature increment, indicating that the LIW salinity increase could also be a factor to be taken into account. Copyright 2005 by the American Geophysical Union.Projects Ecomálaga, Ecobaleares, Ecomurcia, and CIRBAL are supported by the Instituto Español de Oceanografía. L’Estartit station is supported by the Institut de Ciencies del Mar (ICM, CSIC). [...] The May 1998 MATER-I cruise wascarried out in the frame of the project MTPII-MATER: MAS3-CT96-0051, supported by the European UnionPeer Reviewe

Evaluación ambiental de causalidad en el área de infuencia de los pasivos ambientales de origen minero en el río San Juan y delta Upamayo, distritos Simón Bolívar, Tinyahuarco, Vicco, provincia y departamento Pasco, en el 2020

La presente evaluación ambiental de causalidad en el área de influencia de los pasivos ambientales de origen minero en el río San Juan y delta Upamayo (en adelante, EAC PRSJDU) tiene por finalidad, identificar posibles fuentes de alteración en el área de influencia de los pasivos ambientales de origen minero en el río San Juan y delta Upamayo, así como, contribuir a la solución de la problemática socioambiental de la zona (relacionadas a las denuncias ambientales registradas) y en cumplimiento del encargo de la DSEM. La presente EAC PRSJDU contiene los resultados, análisis y conclusiones obtenidas de la evaluación ambiental de la calidad de agua, sedimento, suelo, comunidades hidrobiológicas, la geoquímica de roca y residuos mineros, así como de los estudios de levantamiento fotogramétrico y niveles de toxicidad de sedimento y agua superficial; la cual constituirá un soporte técnico para las acciones de supervisión y fiscalización ambiental del OEFA, y formarán parte del expediente N.º 193-2020-DSEM-CMIN. La evaluación ambiental de causalidad constó de 3 etapas: la primera fue la planificación de la evaluación, que consistió en la definición de los objetivos, revisión de la información relevante, coordinaciones con otras entidades públicas; la segunda fue la ejecución, la cual radicó en el desarrollo de acciones técnicas9 en el área de influencia de los pasivos ambientales de origen minero en el río San Juan y delta Upamayo, para eso se ejecutaron 2 salidas a campo; finalmente, la tercera etapa comprendió los resultados, que está relacionado al análisis y procesamiento de la información recopilada en las etapas previas

The transcriptional and mutational landscapes of lipid metabolism-related genes in colon cancer

Metabolic alterations encountered in tumors are well recognized and considered as a hallmark of cancer. In addition to Warburg Effect, epidemiological and experimental studies support the crucial role of lipid metabolism in colorectal cancer (CRC). The overexpression of four lipid metabolism-related genes (ABCA1, ACSL1, AGPAT1 and SCD genes) has been proposed as prognostic marker of stage II CRC (ColoLipidGene signature). In order to explore in depth the transcriptomic and genomic scenarios of ABCA1, ACSL1, AGPAT1 and SCD genes, we performed a transcriptomic meta-analysis in more than one thousand CRC individuals. Additionally we analyzed their genomic coding sequence in 95 patients, to find variants that could orchestrate CRC prognosis. We found that genetic variant rs3071, located on SCD gene, defines a 9.77% of stage II CRC patients with high risk of death. Moreover, individuals with upregulation of ABCA1 and AGPAT1 expression have an increased risk of CRC recurrence, independently of tumor stage. ABCA1 emerges as one of the main contributors to signature's prognostic effect. Indeed, both high ABCA1 expression and presence of tumoral genetic variants located in ABCA1 coding region, seem to be associated with CRC risk of death. In addition the non-synonymous polymorphism rs2230808, located on ABCA1, is associated with gene expression. Patients carrying at least one copy of minor allele showed higher levels of ABCA1 expression than patients carrying homozygous major allele. This study broaden the prognostic value of ABCA1, ACSL1, AGPAT1 and SCD genes, independently of CRC tumor stage, leading to future precision medicine approaches and "omics"-guided therapiesMinisterio de Economía y Competitividad del Gobierno de España (MINECO, Plan Nacional I+D+i AGL2016-76736-C3), Gobierno regional de la Comunidad de Madrid (P2013/ABI-2728, ALIBIRD-CM) and EU Structural Fund

The biogeochemical context of marine planktonic ecosystems

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